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Only those wells that contain Bovine CASP8
And then biotinylated Human NKX2-5 Antibody is added and binds to NKX2-5 in the sample
it binds with TIMP-3 bound to precoated antibody
a capture antibody-DCLK1-biotin-detection antibody complex was formed
Then Streptavidin-HRP is added and binds to the Biotinylated MMP20 antibody
Human SHC/SLP-76 (SH2-Containing Protein) ELISA Kit Packaging:48 wells Only those wells that containThe Human SHC SLP 76 (SH2 Containing Protein) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human SHC SLP 76. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SHC SLP 76 and Avidin Horseradish Peroxidase (HRP) conjugate are added
