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The assay plate has been pre-coated with mouse anti-Ferret CD8 alpha monoclonal antibody
And then biotinylated Human ARHGDIB Antibody is added and binds to ARHGDIB in the sample
Substrate solution is then added and color develops in proportion to the amount of Human CD84
And then biotinylated Human CA12 Antibody is added and binds to CA12 in the sample
The concentration of Rat PEBP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Human GPA33 (Cell Surface A33 Antigen) ELISA Kit Packaging:96 wells The assay plate has beenThe Human (GPA33) Cell Surface A33 Antigen ELISA Kit measures Cell surface A33 antigen in samples. The plate has been pre coated with Human GPA33 antibody. GPA33 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GPA33 Antibody is added and binds to GPA33 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GPA33 antibody. After incubation unbound Streptavidin HRP is washed
