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FLOT1 present in the sample is added and binds to antibodies coated on the wells
The concentration of Human EFNB2 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated CIDEB antibody
Then a biotinylated detection antibody specific for Human IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
IL12B present in the sample is added and binds to antibodies coated on the wells
Rat LIF (Leukemia Inhibitory Factor) ELISA Kit Packaging:96 wells FLOT1 present in the sampleThe Rat (LIF) Leukemia Inhibitory Factor ELISA Kit measures leukemia inhibitory factor in Rat samples. The plate has been pre coated with Rat LIF antibody. LIF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LIF Antibody is added and binds to LIF in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LIF antibody. After incubation unbound Streptavidin HRP is washed away during
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