Rat ICOS (Inducible T-cell costimulator) ELISA Kit Packaging:96 wells Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Human GRP78 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

If the sample contains ESM1

The concentration of Human SHH in the samples is then determined by comparing the OD of the samples to the standard curve

And then biotinylated Mouse WFDC2 Antibody is added and binds to WFDC2 in the sample

Calculate the concentration of KISS1 in the sample by plotting standard curve

Rat ICOS (Inducible T-cell costimulator) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Rat ICOS (Inducible T cell costimulator) ELISA Kit measures ICOS in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ICOS antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated