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Substrate solution is then added and color develops in proportion to the amount of Rabbit IFNG
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRAF5
The concentration of Human STOML2 in the samples is then determined by comparing the OD of the samples to the standard curve
only those wells that contain Human HVEM
When the sample containing CEACAM6 is added to the plate
Rabbit CASP3 (Caspase 3) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rabbit (CASP3) Caspase 3 ELISA Kit measures Caspase 3 in Rabbit samples. The plate has been pre coated with Rabbit CASP3 antibody. CASP3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rabbit CASP3 Antibody is added and binds to CASP3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CASP3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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