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Then a biotinylated detection antibody specific for Human NAGase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
a horseradish peroxidase conjugated mouse anti-Human LOXL2 Antibody is added to the wells and binds to LOXL2 in the sample
only those wells that contain Human COL1A1
The concentration of Rat LGALS3BP in the samples is then determined by comparing the OD of the samples to the standard curve
You can calculate the concentration of Rat PCT in the samples by comparing the OD of the samples to the standard curve
Rat RLN (Relaxin) ELISA Kit (Small Sample) Packaging:48 wells Then a biotinylated detection antibodyThe Rat RLN (Relaxin) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat RLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat RLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those
