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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CSN3
Calculate the concentration of CD226 in the sample by plotting standard curve
The assay plate has been pre-coated with mouse anti-Mouse IL-18Rα monoclonal antibody
Then Streptavidin-HRP is added and binds to the Biotinylated ENTPD4 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated PSMC4 antibody
Human ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit INCAM-100 Standards or samples are addedThe Human ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit measures ESM1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ESM1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
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