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The concentration of Human SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Porcine GHRL antibody
Then a biotinylated detection antibody specific for Human COL3α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Human SPI1 antibody
FZD4 present in the sample is added and binds to antibodies coated on the wells
Goat/Sheep PTMA (Prothymosin alpha) ELISA Kit Packaging:96 wells The concentration of Human SIRT1The Goat Sheep PTMA (Prothymosin alpha) ELISA Kit measures PTMA in Goat Sheep Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PTMA antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
