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The concentration of OPG in the sample was calculated by drawing a standard curve
it binds with NAIF1 bound to precoated antibody
it binds with VKORC1 bound to precoated antibody
Substrate solution is then added and color develops in proportion to the amount of Human IDE
Then a biotinylated detection antibody specific for Human CVL/EZR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Mouse High Sensitivity IL-1Beta (Interleukin 1 Beta) ELISA Kit beta-D-N-acetylhexosaminidase The concentration of OPG inThe Mouse High Sensitivity IL 1Beta (Interleukin 1 Beta) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse IL 1Beta. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL 1Beta and Avidin Horseradish Peroxidase (HRP) conjugate are added
