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Then a biotinylated detection antibody specific for Human HS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NGF
only those wells that contain Mouse PPARa
and the color intensity is directly proportional to the amount of Human interferon-alpha present
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPP2
Canine REN (Renin) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Canine (REN) Renin ELISA Kit measures Renin in Canine samples. The plate has been pre coated with Canine Renin antibody. Renin present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine Renin Antibody is added and binds to Renin in the sample. Then Streptavidin HRP is added and binds to the Biotinylated Renin antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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