Mouse SEMA3B (Semaphorin-3B) ELISA Kit P14 The micro ELISA plate provided

The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat cTnT/TNNT2

The concentration of Mouse GDF2 in the samples is then determined by comparing the OD of the samples to the standard curve

Substrate solution is then added and color develops in proportion to the amount of Human BHMT

And then biotinylated Human CRTAP Antibody is added and binds to CRTAP in the sample

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NAP

Mouse SEMA3B (Semaphorin-3B) ELISA Kit P14 The micro ELISA plate providedThe Mouse (SEMA3B) Semaphorin 3B ELISA Kit measures Semaphorin 3B in Mouse samples. The plate has been pre coated with Mouse SEMA3B antibody. SEMA3B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SEMA3B Antibody is added and binds to SEMA3B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SEMA3B antibody. After incubation unbound Streptavidin HRP is washed away during