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Substrate solution is then added and color develops in proportion to the amount of Mouse CSPG4
The concentration of Guinea pig IL8 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated PFDN4 antibody
The concentration of Rat C4 in the samples is then determined by comparing the OD of the samples to the standard curve
When the sample containing CD34 is added to the plate
Rat APOB (Apolipoprotein B) ELISA Kit HLP Substrate solution is then addedThe Rat APOB (Apolipoprotein B) ELISA Kit measures Apob in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Apob antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
