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And then biotinylated Mouse IRF4 Antibody is added and binds to IRF4 in the sample
Human β-EP in the sample or standard competes with a fixed amount of Human β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human β-EP
Then a biotinylated detection antibody specific for Rat CT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
H4C1 present in the sample is added and binds to antibodies coated on the wells
NPM1 present in the sample is added and binds to antibodies coated on the wells
Mouse ANG (Angiogenin) ELISA Kit angiopoietin like And then biotinylated Mouse IRF4The Mouse (ANG) Angiogenin ELISA Kit measures Angiogenin in Mouse samples. The plate has been pre coated with Mouse ANG antibody. ANG present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ANG Antibody is added and binds to ANG in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ANG antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
