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Substrate solution is then added and color develops in proportion to the amount of Rat FMO3
Only those wells that contain Mouse ANGPTL2
The concentration of Cattle IP10 in the samples is then determined by comparing the OD of the samples to the standard curve
a capture antibody-CLU-biotin-detection antibody complex was formed
The Mouse IL-33 ELISA kit is a sandwich enzyme immunoassay
Sheep PRF1 (Perforin 1) ELISA Kit P19 Substrate solution is then addedThe Sheep PRF1 (Perforin 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Sheep PRF1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Sheep PRF1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well