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Substrate solution is then added and color develops in proportion to the amount of Human NPTN
The concentration of Mouse BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse GAL7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Human ACTBL1 antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CNP
Human PD-1/PDCD1 (Programmed Cell Death Protein 1) QuickTest ELISA Kit CKM Substrate solution is then addedThe Human PD 1 PDCD1 (Programmed Cell Death Protein 1) QuickTest ELISA Kit measures PDCD1 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and