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LH in the sample or standard competes with a fixed amount of biotin-labeled LH for sites on a pre-coated antibody specific to LH
And then biotinylated Porcine HMOX1 Antibody is added and binds to HMOX1 in the sample
The OD value is proportional to the concentration of Chicken EL
CPSF1 present in the sample is added and binds to antibodies coated on the wells
You can calculate the concentration of Rat GROBeta/CXCL1 in the samples by comparing the OD of the samples to the standard curve
Chicken PEDF (Pigment Epithelium Derived Factor) ELISA Kit Packaging:48 wells LH in the sample orThe Chicken PEDF (Pigment Epithelium Derived Factor) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Chicken PEDF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Chicken PEDF and Avidin Horseradish Peroxidase (HRP) conjugate are added
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