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The optical density(OD) is measured spectrophotometrically at a wavelength of 450±2 nm
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAX6
it binds with APPL2 bound to precoated antibody
APLN present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Rat VEGFR-1 Antibody is added and binds to VEGFR-1 in the sample
Human PROPSA (precursor of Prostate Specific Antigen) ELISA Kit Packaging:96 wells The optical density(OD) is measuredThe Human PROPSA (precursor of Prostate Specific Antigen) ELISA Kit measures proPSA in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti proPSA antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
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