Rat AQP-4 (Aquaporin 4) ELISA Kit Chemokine(C-X-C motif) ligand 9 VIP present in the sample

VIP present in the sample is added and binds to antibodies coated on the wells

Then a biotinylated detection antibody specific for Human NAGase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

and the Human LpPLA2 standard plate wells that pre-coated using protein-related techniques are provided separately

Then a biotinylated detection antibody specific for Human EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GP39

Rat AQP-4 (Aquaporin 4) ELISA Kit Chemokine(C-X-C motif) ligand 9 VIP present in the sampleThe Rat (AQP 4) Aquaporin 4 ELISA Kit measures Aquaporin 4 in Rat samples. The plate has been pre coated with Rat AQP4 antibody. AQP4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat AQP4 Antibody is added and binds to AQP4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated AQP4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.