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MOTS-c in the sample or standard competes with a fixed amount of biotin-labeled MOTS-c for sites on a pre-coated antibody specific to MOTS-c
You can calculate the concentration of Rat BRAK/CXCL14 in the samples by comparing the OD of the samples to the standard curve
And then biotinylated Human FOXO4 Antibody is added and binds to FOXO4 in the sample
GLUD1 present in the sample is added and binds to antibodies coated on the wells
a capture antibody-LGALS3BP-biotin-detection antibody complex was formed
Porcine ECE1 (Endothelin Converting Enzyme 1) ELISA Kit Corticosterone MOTS-c in the sample orThe Porcine ECE1 (Endothelin Converting Enzyme 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Porcine ECE1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine ECE1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively
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