Human AR (Androgen receptor) ELISA Kit LDLCQ4 Unbound avidin-HRP is washed away

Unbound avidin-HRP is washed away during a washing step

a horseradish peroxidase conjugated mouse anti-Human BCAM Antibody is added to the wells and binds to BCAM in the sample

a horseradish peroxidase conjugated mouse anti-Human PAI-1 Antibody is added to the wells and binds to PAI-1 in the sample

And then biotinylated Human KIND3 Antibody is added and binds to KIND3 in the sample

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCGB2A2

Human AR (Androgen receptor) ELISA Kit LDLCQ4 Unbound avidin-HRP is washed awayThe Human AR (Androgen receptor) ELISA Kit measures AR in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti AR antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.