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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KL-6
the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of pregnenolone present
The Mouse VAP-1 (VascularAdhesion Protein 1) ELISA Kit is a Sandwich enzyme immunoassay
The Chicken IL-12 ELISA kit is a sandwich enzyme immunoassay
Isotype: IgG1
Human ANXA5 (Annexin A5) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (ANXA5) Annexin A5 ELISA Kit measures Annexin A5 in samples. The plate has been pre coated with Human ANXA5 antibody. ANXA5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ANXA5 Antibody is added and binds to ANXA5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ANXA5 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
