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And then biotinylated Canine TIMP1 Antibody is added and binds to TIMP1 in the sample
a horseradish peroxidase conjugated mouse anti-Mouse IL-6R Antibody is added to the wells and binds to IL-6R in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GAL4
MX2 present in the sample is added and binds to antibodies coated on the wells
The OD value is proportional to the concentration of Mouse EGF
Human AKT1 (Rac-alpha Serine, Threonine-protein Kinase) ELISA Kit Packaging:96 wells And then biotinylated Canine TIMP1The Human (AKT1) Rac alpha Serine, Threonine protein Kinase ELISA Kit measures Rac Alpha Serine Threonine Protein Kinase in samples. The plate has been pre coated with Human AKT1 antibody. AKT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human AKT1 Antibody is added and binds to AKT1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated AKT1 antibody. After incubation unbound
