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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSH
Then a biotinylated detection antibody specific for Mouse IL-11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Mouse PINP
The concentration of Human IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve
The Mouse CKMB (Creatine Kinase MB Isoenzyme) ELISA Kit is a Sandwich enzyme immunoassay
Human ODF4 (Outer Dense Fiber Protein 4) ELISA Kit TNFR55 Standards or samples are addedThe Human (ODF4) Outer Dense Fiber Protein 4 ELISA Kit measures Outer Dense Fiber Protein 4 in samples. The plate has been pre coated with Human ODF4 antibody. ODF4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ODF4 Antibody is added and binds to ODF4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ODF4 antibody. After incubation unbound Streptavidin HRP is washed
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