Human WNT5B (Protein Wnt-5B) ELISA Kit Packaging:96 wells Samples (or Standards) and Horseradish

Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG3 are added to the micro ELISA plate wells

Substrate solution is then added and color develops in proportion to the amount of Human SPARC

The OD value is proportional to the concentration of Human CHEM

The OD value is proportional to the concentration of Mouse NOS2/iNOS

You can calculate the concentration of Rat SDF-1/CXCL12 in the samples by comparing the OD of the samples to the standard curve

Human WNT5B (Protein Wnt-5B) ELISA Kit Packaging:96 wells Samples (or Standards) and HorseradishThe Human (WNT5B) Protein Wnt 5B ELISA Kit measures Protein Wnt 5B in samples. The plate has been pre coated with Human WNT5B antibody. WNT5B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human WNT5B Antibody is added and binds to WNT5B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated WNT5B antibody. After incubation unbound Streptavidin HRP is washed away during a washing