Human IAA (Insulin Autoantibodies) ELISA Kit Packaging:96 wells the TMB substrate (enzyme substrate)

the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of total estrogens present

And then biotinylated Mouse GSN Antibody is added and binds to GSN in the sample

The concentration of Rat HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve

The concentration of GZMM in the sample was calculated by drawing a standard curve

And then biotinylated Human GMPS Antibody is added and binds to GMPS in the sample

Human IAA (Insulin Autoantibodies) ELISA Kit Packaging:96 wells the TMB substrate (enzyme substrate)The Human IAA (Insulin Autoantibodies) ELISA Kit measures IAA in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP