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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human I-PTH
Then a biotinylated detection antibody specific for Human ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
ASB9 present in the sample is added and binds to antibodies coated on the wells
NAD subunit alpha
Then a biotinylated detection antibody specific for Human ANGPTL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human SPON1 (Spondin-1) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Human (SPON1) Spondin 1 ELISA Kit measures Spondin 1 in samples. The plate has been pre coated with Human SPON1 antibody. SPON1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SPON1 Antibody is added and binds to SPON1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SPON1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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