Human DHFR (Dihydrofolate reductase) ELISA Kit Sonic hedgehog Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Human SOD1

CHRDL1 present in the sample is added and binds to antibodies coated on the wells

Then Streptavidin-HRP is added and binds to the Biotinylated APBA2 antibody

Then Streptavidin-HRP is added and binds to the Biotinylated CXCL12 antibody

Calculate the concentration of AOPEP in the sample by plotting standard curve

Human DHFR (Dihydrofolate reductase) ELISA Kit Sonic hedgehog Substrate solution is then addedThe Human DHFR (Dihydrofolate reductase) ELISA Kit measures DHFR in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti DHFR antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection