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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDGFBB
The plate has been pre-coated with Mouse ATG4B antibody
a horseradish peroxidase conjugated mouse anti-Human IL17 Antibody is added to the wells and binds to IL17 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated NOTCH1 antibody
The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of ANGPTL-4 in the specimen
Human BAX (Apoptosis regulator BAX) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human BAX (Apoptosis regulator BAX) ELISA Kit measures BAX in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti BAX antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
