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The plate has been pre-coated with Mouse GAL3 antibody
And then biotinylated Rat GPC3 Antibody is added and binds to GPC3 in the sample
And then biotinylated Human MOCOS Antibody is added and binds to MOCOS in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Pro-MMP-9
Then a biotinylated detection antibody specific for CD5L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human DEFB129 (Beta-defensin 129) ELISA Kit Packaging:96 wells The plate has been pre-coatedThe Human DEFB129 (Beta defensin 129) ELISA Kit measures DEFB129 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti DEFB129 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
