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ANG-2 present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated ATP1A2 antibody
And then biotinylated Bovine ANXA1 Antibody is added and binds to ANXA1 in the sample
The standards and pilot samples were added to the wells subsequently
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MIP3a
Human C1INH (Complement 1 Inhibitor) QuickTest ELISA Kit Packaging:96 wells ANG-2 present in the sampleThe Human C1INH (Complement 1 Inhibitor) QuickTest ELISA Kit measures C1INH in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples
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