sST2 (Soluble ST2) ELISA Kit (Mini-Sample) ACEE The substrate solution is added

The substrate solution is added to each well

The Bovine Adiponectin ELISA kit is a sandwich enzyme immunoassay

The Mouse Tumor Necrosis Factor Alpha kit is a Sandwich immunoassay

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken TNFa

The OD value is proportional to the concentration of Rat AGER

sST2 (Soluble ST2) ELISA Kit (Mini-Sample) ACEE The substrate solution is addedThis sST2 (Soluble ST2) ELISA Kit (Mini Sample) uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to sST2 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for sST2 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated.