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The Mouse HMGB-1 (High Mobility Group Protein B1) ELISA Kit is a Sandwich enzyme immunoassay
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues
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Then Streptavidin-HRP is added and binds to the Biotinylated GRM4 antibody
And then biotinylated Rat CALR Antibody is added and binds to CALR in the sample
Human ENDOG (Endonuclease G) ELISA Kit Packaging:96 wells The Mouse HMGB-1 (High MobilityThe Human ENDOG (Endonuclease G) ELISA Kit measures ENDOG in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ENDOG antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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