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The OD value is proportional to the concentration of Porcine IL-1Beta
Substrate solution is then added and color develops in proportion to the amount of Human ASPM
a horseradish peroxidase conjugated mouse anti-Human LAYN Antibody is added to the wells and binds to LAYN in the sample
Only those wells that contain NGAL
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C4
Human CLDN14 (Claudin 14) ELISA Kit Packaging:96 wells The OD value is proportionalThe Human (CLDN14) Claudin 14 ELISA Kit measures Claudin 14 in samples. The plate has been pre coated with Human CLDN14 antibody. CLDN14 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CLDN14 Antibody is added and binds to CLDN14 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CLDN14 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
