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cath-D present in the sample is added and binds to antibodies coated on the wells
The concentration of Human MIP4a in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Human TMEM180
And then biotinylated Mouse PNPLA2 Antibody is added and binds to PNPLA2 in the sample
And then biotinylated Rat CX3CR1 Antibody is added and binds to CX3CR1 in the sample
Bovine IL-10 (Interleukin 10) ELISA Kit Packaging:96 wells cath-D present in the sampleThe Bovine IL 10 (Interleukin 10) ELISA Kit measures IL 10 in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 10 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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