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Then a biotinylated detection antibody specific for RANκL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Discreet design
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PC
Mouse β-EP in the sample or standard competes with a fixed amount of Mouse β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-EP
The Human (CNN2) Calponin 2 ELISA Kit measures Calponin 2 in samples
Mouse MUC19 (Mucin-19) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Mouse (MUC19) Mucin 19 ELISA Kit measures Mucin 19 in Mouse samples. The plate has been pre coated with Mouse MUC19 antibody. MUC19 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse MUC19 Antibody is added and binds to MUC19 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MUC19 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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