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Then Streptavidin-HRP is added and binds to the Biotinylated DNM2 antibody
Then a biotinylated detection antibody specific for Mouse TPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Human GDNF
You can calculate the concentration of Mouse sIgA in the samples by comparing the OD of the samples to the standard curve
Calculate the concentration of sSELP in the sample by plotting standard curve
Human VCAM1 (Vascular cell adhesion protein 1) QuickTest ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Human VCAM1 (Vascular cell adhesion protein 1) QuickTest ELISA Kit measures VCAM1 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot
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