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Substrate solution is then added and color develops in proportion to the amount of Bovine NANOG
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TLR10
Only those wells that contain Human IL-5
Then Streptavidin-HRP is added and binds to the Biotinylated CENPK antibody
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRAK
Rat CNTN1 (Contactin-1) ELISA Kit HBGF-1 Substrate solution is then addedThe Rat (CNTN1) Contactin 1 ELISA Kit measures Contactin 1 in Rat samples. The plate has been pre coated with Rat CNTN1 antibody. CNTN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CNTN1 Antibody is added and binds to CNTN1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CNTN1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
