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The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 54 nm
Then a biotinylated detection antibody specific for IFN-Gamma and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The Mouse EGFR (Epidermal Growth Factor Receptor) ELISA kit uses the Sandwich-ELISA principle
The OD value is proportional to the concentration of Mouse GROalpha/CXCL1
only those wells that contain Mouse MYH7
Rat ADORA2a (Adenosine A2a Receptor) ELISA Kit (Small Sample) PC-I The optical density (OD) isThe Rat ADORA2a (Adenosine A2a Receptor) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat ADORA2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat ADORA2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
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