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The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody
it binds with GADD45A bound to precoated antibody
The concentration of Rat GP73 in the samples is then determined by comparing the OD of the samples to the standard curve
RAB3B present in the sample is added and binds to antibodies coated on the wells
Calculate the concentration of EIF3I in the sample by plotting standard curve
Human OPTC (Opticin) ELISA Kit Packaging:96 wells The specimen is washed outThe Human OPTC (Opticin) ELISA Kit measures OPTC in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti OPTC antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then,
