Human CALB (Calbindin) ELISA Kit RO Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CHRDL2

Then a biotinylated detection antibody specific for Human BAX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The concentration of Rat CTSB in the samples is then determined by comparing the OD of the samples to the standard curve

The concentration of Human ATG7 in the samples is then determined by comparing the OD of the samples to the standard curve

Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light

Human CALB (Calbindin) ELISA Kit RO Standards or samples are addedThe Human (CALB) Calbindin ELISA Kit measures Calbindin in samples. The plate has been pre coated with Human CALB1 antibody. CALB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CALB1 Antibody is added and binds to CALB1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CALB1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.