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the Mouse HIF-1Alpha (Hypoxia Inducible Factor 1 Alpha) ELISA kit uses the Sandwich-ELISA principle
You can calculate the concentration of Human ADAMTS4 in the samples by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Human VEGF-A
You can calculate the concentration of Human CALP in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Rat COMP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat VIP (Vasoactive Intestinal Peptide) ELISA Kit TNFR60 the Mouse HIF-1Alpha (Hypoxia InducibleThe Rat VIP (Vasoactive Intestinal Peptide) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Rat VIP. During the reaction, Rat VIP in the sample or standard competes with a fixed amount of Rat VIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat VIP. Excess conjugate and unbound sample or standard are washed away, and Avidin Horseradish Peroxidase
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