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a horseradish peroxidase conjugated mouse anti-Human Marapsin Antibody is added to the wells and binds to Marapsin in the sample
coli expression system and the target gene encoding Met1-Leu166 is expressed with a 6His tag at the N-terminus
Then a biotinylated detection antibody specific for Human FGF18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated ATOH1 antibody
The OD value is proportional to the concentration of Human TIMP-4
Mouse RSPO3 (R-Spondin-3) ELISA Kit Packaging:96 wells a horseradish peroxidase conjugated mouseThe Mouse (RSPO3) R Spondin 3 ELISA Kit measures R spondin 3 in Mouse samples. The plate has been pre coated with Mouse RSPO3 antibody. RSPO3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse RSPO3 Antibody is added and binds to RSPO3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RSPO3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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