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Calculate the concentration of C/EBPα in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated ROCK1 antibody
HNF1A present in the sample is added and binds to antibodies coated on the wells
The concentration of Mouse MT in tested samples can be calculated by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse C4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human PGF2ALPHA (Prostaglandin F2Alpha) ELISA Kit SCYB4 Calculate the concentration of C/EBPαThe Human PGF2ALPHA (Prostaglandin F2Alpha) ELISA Kit measures PGF2Alpha in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with PGF2. PGF2 in the sample or standard competes with a fixed amount of biotin labeled PGF2 for sites on a pre coated antibody specific to PGF2. Free components are washed
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