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The concentration of Human pPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TNFa
the Porcine IL-6 (Interleukin 6) ELISA kit uses the Sandwich-ELISA principle
Then a biotinylated detection antibody specific for Human GAL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Bovine AFP Antibody is added and binds to AFP in the sample
Human ASS1 (Argininosuccinate Synthase) ELISA Kit Packaging:96 wells The concentration of Human pPLA2The Human (ASS1) Argininosuccinate Synthase ELISA Kit measures Argininosuccinate Synthase in samples. The plate has been pre coated with Human ASS1 antibody. ASS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ASS1 Antibody is added and binds to ASS1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ASS1 antibody. After incubation unbound Streptavidin HRP is washed away
