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Then Streptavidin-HRP is added and binds to the Biotinylated GLG1 antibody
a capture antibody-MTHFD1L-biotin-detection antibody complex was formed
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And then biotinylated Mouse ATG5 Antibody is added and binds to ATG5 in the sample
SPARCL1 in the sample or standard competes with a fixed amount of biotin-labeled SPARCL1 for sites on a pre-coated antibody specific to SPARCL1
Rat INSL3 (Insulin-like 3) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Rat INSL3 (Insulin like 3) ELISA Kit measures Insl3 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Insl3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.